β-glucosidase and cyclodextrin glucanotransferase (CGTase). Capsaicin and Ltd. and CGTase was from Amano Pharmaceutical. Co. Ltd. Mice were
of CGTase (CGTase I.2 0, p1-I 5.8, temperature 4O”C, incubation time 2 h) [7] and analyzed by HPLC using a TSK gel amide XOcolumn Published by Elsevier Science Publishers B. V. 13
CGTase (Paenibacillus macerans) was purchased from Amano Enzyme Inc. Resveratrol 3-maltoside (8) and resveratrol 4′-β-maltoside (9) were produced individually as fol-lows. To a solution containing resveratrol 3-β-glucoside (2) … In our work, the CGTase from Thermoanaerobacter sp. (Tor- uzyme 3.0 L) gave a higher yield than that from Bacil- lus macerans (CGTase Amano). In contrast with the CGTase from Bacillus macerans (the HPLC chroma- togram showed four different compounds with in- creasing concentration, indicating the formation of the so-called analogous series (A) reaction of DGAS, (B) reaction of CGTase (Amano Co.). Mass spectra were obtained by Single Ion Recording (SIR) in positive mode. The fragment ions were appeared owing to the analysis condition of HPLC/QDa, accordingly, DA3 (m/z+ 741) showed its fragment (m/z+ 417) by reduction of molecular weight of two glucosyl (MW 162 × 2) moieties. Amano Enzyme, Inc. (Japan), and was used without further purifi-cation.
To this was added 15 g cyclodextrin glucanotransferase (CGTase, Amano Enzyme Inc., product name: Contizyme, 600 U/mL) and a reaction was started, followed by holding for 24 hours at pH 7.25 and 60° C. Abstract Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8wt%) solution were mixed followed by electrospinning (−9kV, 3h). CGTase/PVA nanofibres with an average diameter of 176±46nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. The synthesis of curcumin oligosaccharides was performed by incubating the reaction mixture (10 mL) containing 0.2 mmol of curcumin d-glucoside, 5 g of soluble starch, and 200 units of CGTase from Bacillus macerans purchased from Amano Pharmaceutical Co. Ltd. in 25 mM sodium phosphate buffer (pH 7.0) at 40 °C for 24 hours. CGTase derived from Bacillus macerans was obtained as a kind gift from Amano Enzyme, Inc., Nagoya, Japan, in a stock solution that was used as supplied and stored at 5 °C. Water was purified on a Merck Millipore Synergy UV water purification system prior to use.
(Toruzyme 3.0L) worked better than that from Bacillus macerans (CGTase Amano).
Chemicals (St. Louis, Mo.). Cyclomaltodextrin glucanotransferase (CGTase) (from Paenibacillus macerans) was provided by Amano Enzyme Inc. (Nagoya, Japan). All other chemicals used were from commercial sources and were ana-lytical grade. Microorganisms and medium. V. dahliae TPU 4900, Bacillus cereus TPU 5504,
CGTase (Paenibacillus macerans) was purchased from Amano Enzyme Inc. Resveratrol 3-maltoside (8) and resveratrol 4′-β-maltoside (9) were produced individually as fol-lows. To a solution containing resveratrol 3-β-glucoside (2) … In our work, the CGTase from Thermoanaerobacter sp. (Tor- uzyme 3.0 L) gave a higher yield than that from Bacil- lus macerans (CGTase Amano).
Bacillus macerans CGTase, here called Amano, (EC.2.4.1.19) was kindly provided by Amano enzyme Europe Ltd. (Milton Keynes, UK) and Thermoanaerobacter sp. ATCC 53627 CGTase (Toruzyme 3.0L,) from Novozymes (Bagsvaerd, Denmark).
The transglycosylation activity of CGTase is well reported, as it is able to glucosylate other phenolic compounds such The enzyme from Thermoanaerobacter sp. (Toruzyme 3.0L) worked better than that from Bacillus macerans (CGTase Amano). The transglycosylation activity of CGTase is well reported, as it is able to glucosylate other phenolic compounds such as resveratrol [ 26, 27 ], catechin [ 28 ], hydroquinone [ 29 ], kaempferol [ 30] or genistein [ 31 ]. Table 1. Cyclodextrin glucanotransferase (CGTase) from Bacillus macerans was applied to produce the CDs from linear α- (1,4)-glucans, which were obtained by Neisseria polysaccharea amylosucrase (Np AS) treatment on sucrose. The greatest CD yield (21.1%, w/w) was achieved from a one-pot dual enzyme reaction at 40 °C for 24 h.
Soluble starch was purchased from Yakuri Pure Chemicals Co., Ltd. (Osaka, Japan). CGTases belong to family 13 of glycoside hydrolases, the large group of α-amylase family enzymes, which also include α-amylases, isoamylases, amylopullulanases, pullulanases, neopullulanases, and branching enzymes. 13 Despite their structural similarity with other enzymes in the family catalyzing hydrolysis and/or glycosyl-transfer of α (1→4) and α (1→6)-glycosidic linkages, CGTases have been reported to mainly catalyze α (1→4)-glycosyl transfer reactions. Amano Enzyme was founded 120 years ago in Japan as a pharmaceutical business, expanding into specialty enzymes in 1948 with our first item—malt diastase. Today, we have grown into one of the top enzyme manufacturers, producing enzyme solutions for any industry and every need.
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Publisher Summary This chapter discusses the purification and action of cyclodextrin-producing enzyme (CGTase). Purification of macerans CGTase can be accomplished by following steps: Starch adsorption and desorption, column chromatography on diethylaminoethyl-cellulose, and crystallization. Cyclodextrin glycosyltransferases (CGTases) from Paenibacillus macerans, Thermoanaerobacter sp. ATCC 53627, Bacillus stearothermophilus and a Carboxydocella sp. (phylogenetically identified from genomic DNA) were characterized with respect to their catalytic activity in different reactions, with emphasis on reactions useful for the elongation of the carbohydrate group of alkyl glycosides.
Publisher Summary This chapter discusses the purification and action of cyclodextrin-producing enzyme (CGTase). Purification of macerans CGTase can be accomplished by following steps: Starch adsorption and desorption, column chromatography on diethylaminoethyl-cellulose, and crystallization. Cyclodextrin glycosyltransferases (CGTases) from Paenibacillus macerans, Thermoanaerobacter sp. ATCC 53627, Bacillus stearothermophilus and a Carboxydocella sp.
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Chemicals (St. Louis, Mo.). Cyclomaltodextrin glucanotransferase (CGTase) (from Paenibacillus macerans) was provided by Amano Enzyme Inc. (Nagoya, Japan). All other chemicals used were from commercial sources and were ana-lytical grade. Microorganisms and medium. V. dahliae TPU 4900, Bacillus cereus TPU 5504,
The enzyme was eluted with a linear gradient between 0 and 0.5 M NaCl in Tris–HCl buffer, pH 7.8. この物質をつくる酵素をサイクロデキストリングルカノトランスフェラーゼ（CGTase）といいます。 そこに空間があるから。 この輪の中には、何が入れられるでしょうか？ studies on glycosyltransferases other than CGTase, we are interested in the action of CGTase in producing cyclic glucans larger than CDs. In this paper, we investigated the initial action of CGTase from an alkalophilic Bacillus sp.
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The crude CGTase extract with the corn starch substrate showed a productivity of 0.38 mmol/L/h, which was 29 % lower than using the purified enzyme and the corn starch substrate but 7 % higher
The enzyme from Thermoanaerobacter sp. (Toruzyme 3.0L) worked better than that from Bacillus macerans (CGTase Amano). The transglycosylation activity of CGTase is well reported, as it is able to glucosylate other The crude CGTase extract with the corn starch substrate showed a productivity of 0.38 mmol/L/h, which was 29 % lower than using the purified enzyme and the corn starch substrate but 7 % higher Ethyl acrylate, vinyl propionate, tert-butanol, dioxane, glucose, maltose and lipases from C. antarctica and T. lanuginosus were purchased from Sigma (Steinheim, Germany), α-cyclodextrin from Wacker Chemie AG (Burghausen, Germany), immobilized lipase from C. antarctica (Novozym 435) was obtained from Novozymes (Bagsvaerd, Denmark) and CGTase from B. macerans was obtained from Amano Enzyme CGTase from Bacillus macerans (cyclodextrin glucanotransferase) and α-glucosidase from Aspergillus niger (Transglucosidase L) were kindly supplied by Amano Enzyme Inc. (Oxfordshire, UK). The β-galactosidase from Bacillus circulans (Biolactase NTL Conc. 2x) was provided from Biocon (Barcelona, Spain). Substrate, hesperetin, was purchased from Sigma-Aldrich Co. CGTase was purchased from Amano Pharmaceutical Co. Ltd. The NMR spectra were recorded in CD 3 OD using a Varian XL-400 spectrometer. The chemical shifts were expressed in δ (ppm) referring to tetramethylsilane.
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Samce rosną zwykle do 3,5 cm długości, samice do 4 cm, chociaż spotyka się jeszcze większe osobniki.Ciało przezroczyste, na grzbiecie biegnie linia, na bokac Glucoamylase was from Toyobo Co., Ltd. (Osaka, Japan) and B. macerans CGTase was from Amano Enzyme Inc. (Aichi, Japan). The enzyme was further purified using DEAE Sepharose Fast Flow media (Amersham Biosciences Europe GmbH).
Thermoanaerobacter sp. on Kei Nakagawa, Hiroki Amano, Magnus Persson & Ronny Berndtsson, 2021 dec, and cyclodextrin glucanotransferase (CGTase)‐catalysed transglycosylation. Kei Nakagawa, Hiroki Amano, Magnus Persson & Ronny Berndtsson, 2021 Dec, and cyclodextrin glucanotransferase (CGTase)‐catalysed transglycosylation. CGTase was purchased from Amano Enzyme Europe Ltd (Milton Keynes, UK) and Toruzyme 3.0L was obtained from Novozyme A/S (Bagsvaerd, Denmark). Dodecyl-β-maltooctaoside was produced in-house as described previously (Svensson et al. 2009a).